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. 2021 Nov 22;12:767136. doi: 10.3389/fphys.2021.767136

FIGURE 5.

FIGURE 5

Inhibition of Piezo1 suppressed osteoclastic activities on the tension side. TRAP staining (A) and quantification of TRAP-positive cells (D). TRAP-positive osteoclasts were detected near the tension side of the roots. On day 3, the number of osteoclasts in the OTM group was significantly greater than that of the CON and the OTM + INH groups. Scale bar = 50 μm. Immunohistochemical staining of RANKL (B) and OPG (C) of PDL on the tension side of the distobuccal roots. The means of the integrated optical density (IOD) of RANKL (E), OPG (F), and RANKL/OPG ratio (G) were shown. On day 3, the RANKL/OPG ratio of the OTM group was significantly greater than that of the CON and OTM + INH groups. Western blot analyses (H) were carried out to detect the protein levels of RANKL and OPG on day 3 of OTM. The protein expression of the RANKL/OPG ratio increased significantly in the OTM group and was reduced by GsMTx4 in the OTM + INH group. Data are presented as the mean ± SD. *p < 0.05. Scale bar = 50 μm. TRAP, tartrate-resistant acid phosphatase; RANKL, receptor activator of nuclear factor-kappa B ligand; OPG, osteoprotegerin; AB, alveolar bone; R: root.