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. 2021 Nov 22;12:781108. doi: 10.3389/fimmu.2021.781108

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Copyright © 2021 Hildersley, McNeilly, Gillan, Otto, Löser, Gerbe, Jay, Maizels, Devaney and Britton

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Single cell RNA sequencing (scRNA-seq) identifies distinct ovine abomasal cell clusters. (A) UMAP (Uniform manifold approximation and projection) plot of cell clusters identified from integrated data from sheep 1 and 2 following mapping to bovine genome. DC - dendritic cells, ECL - enterochromaffin-like cells. Cell clusters were putatively identified by expression of marker genes (see Supplementary Table 6 ). Expression of epithelial cell marker EPCAM and immune marker PTPRC (CD45) across all clusters, shown by (B) UMAP and (C) violin plots. (D) UMAP plot of sheep 1 and 2 overlapped. (E) Relative proportion of cells within each cluster for sheep samples 1 and 2.