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. 2021 Nov 22;12:781108. doi: 10.3389/fimmu.2021.781108

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Copyright © 2021 Hildersley, McNeilly, Gillan, Otto, Löser, Gerbe, Jay, Maizels, Devaney and Britton

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Trajectory analysis of ovine abomasal tuft cell sub-clusters. (A) UMAP plot of ovine tuft cell sub-clusters 1, 2, 3. (B) UMAP plot showing gene expression (UMI) across sub-clusters. (C) UMAP plot of sheep 1 and 2 tuft cell sub-clusters overlapped. (D) Violin plots showing log-normalised expression of selected tuft cell genes across sub-clusters. (E) Heatmap of top 10 marker genes in ovine tuft cell sub-clusters (see Supplementary Table 8 for full gene lists). (F) PHATE (Potential of Heat-diffusion for Affinity-based Transition Embedding) plot of sub-cluster gene expression. Arrow on Slingshot line shows suggested direction of lineage, added manually, starting at sub-cluster 1.