Figure 2.

Myoblasts maintain a higher lactic acid secretion rate than myotubes. C2C12 myoblasts and in vitro-differentiated C2C12 myotubes were infected with T. gondii or were left non-infected. Cell culture supernatants were collected at time points as indicated and metabolites were profiled by GC-MS. (A, B) Glucose and lactic acid concentrations were determined in cell culture supernatants of non-infected (ni) and T. gondii-infected (Tg) myoblasts and myotubes between 0 and 72 hours of infection. Data are means ± S.E.M. from three independent experiments; significant differences were identified by ANOVA (**p < 0.01). (C, D) Glucose consumption and lactic acid secretion were calculated during the initial 24 hours of infection and were normalized to total protein. Results are means ± S.E.M. from three independent experiments. Significant differences were identified by Student’s t-test (*p < 0.05).