FIGURE 4.

Analysis of complex IV enzyme activity by high-resolution respirometry. (A) Qualitative data of complex IV enzyme activity were recorded after the addition of ascorbate, TMPD and cytochrome c expressed as the oxygen flux (pmol/s/mill cells) with chemical background correction using the value recorded after the addition of sodium azide. (B) The level of complex IV enzyme activity in SH-SY5Y cells overexpressing different types of APP (WT, Swe, and Swe/Ind) expressed as % of the nontransfected SH-SY5Y control cells (NT). *: p < 0.05, **: p < 0.01. (C) Effects of ATF and GTF treatment on complex IV enzyme activity in nontransfected SH-SY5Y (NT) and cells overexpressing different types of APP (WT, Swe, and Swe/Ind) expressed as % of the untreated group in respective type of cells. *: p < 0.05, **: p < 0.01, ***p < 0.001. Data are expressed as the mean ± SD, (N = 3).