TABLE 3.
REA patterns of 98-MDa plasmids and PFGE analysis of XbaI (5′-TCTAGA-3′)-digested chromosomal DNA
| Isolate origin (no.) | No. of REA fragments from digestion with:
|
REA pattern(s) of plasmids | PFGE pattern(s)a | ||
|---|---|---|---|---|---|
| HindIII | EcoRI | HindIII + EcoRI | |||
| Rawalpindi (25) | 9–10 | 9–10 | 15–18 | I | A1 (18), A2 (3), B1 (4) |
| Quetta (122) | 8–9 | 11–12 | 16–17 | III and IV | A1 (92), B1 (12), B2 (10), E1 (2), E2 (6) |
| Kuwait (8) | 7–8 | 8–9 | 15–18 | II | C3 (8) |
| Bangladesh (30) | 7–8 | 8–9 | 15–18 | II | C1 (30) |
| Malaysia (6) | 7–8 | 8–9 | 15–18 | II | D1 (1), D2 (2), E1 (3) |
| India (2) | 7–8 | 8–9 | 15–18 | II | C2 (2) |
a
The PFGE patterns were arbitrarily designated groups A to E. The pattern for each group differs by more than seven bands from those of the other groups. The subgroup patterns (e.g., C1, C2, C3) differed from each other by fewer than seven bands. Numbers in parentheses are numbers of isolates in the subgroup.