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. 2021 Nov 30;14:6331–6348. doi: 10.2147/JIR.S329230

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© 2021 Zhang et al.

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PLIN2 knock down attenuates RVLM microglial M1 polarization and proliferation in stressed rats. The representative double immunofluorescent image of iNOS (M1 polarization marker) with Iba-1 (A) co-localization and the percent of iNOS⁺/Iba-1⁺ cells (B) in the RVLM of rats. Scale bar = 50 μm. The representative double immunofluorescent image of colocalization of PCNA (proliferation marker) with Iba-1 (C) and the percent of PCNA⁺/Iba-1⁺ cells (D) in the RVLM of rats. Scale bar = 50 μm. The representative immunofluorescent image of IL-1β (upper panel)/ TNF-α (lower panel) (E) and quantification of immunopositive intensity of IL-1β (F)/ TNF-α (G) in the RVLM. Data are presented as the mean ± SD. Scale bar = 50 μm. n = 6–8, *p < 0.05, ^#p < 0.05, t-test.