Table 1.

Example of GFP+ T cell count from coculture

Time min	Raw data cell numbers per FOV
	UT 1	UT 2	UT 3	Sham serum (25%) 1	Sham serum (25%) 2	Sham serum (25%) 3	Stroke serum (25%) 1	Stroke serum (25%) 2	Stroke serum (25%) 3
0:00	401	351	300	398	376	401	423	399	410
30:00	383	336	292	379	351	383	374	351	367
60:00	360	329	263	324	323	354	318	314	300
90:00	348	325	251	293	303	332	271	260	254
120:00	330	310	211	256	245	282	212	220	207
180:00	300	291	200	240	234	263	156	171	104

Data was analyzed using ImageJ software. Microphotographs (Figure 4) from the coculture kinetics were thresholded for GFP+ cells. Thresholded cells were then quantified using “analyze particles” task. All acquired numbers of GFP+ cells were normalized to the according “0:00” cell numbers and plotted in a graph (Figure 4). Abbreviations: UT = untreated