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T cell culture medium
| Reagent | Final concentration | Amount |
|---|---|---|
| RPMI 1640 | N/A | 445 mL |
| FBS | 10% | 50 mL |
| Penicilin/Streptomycin | 1% | 5 mL |
Store at 4°C for a maximum of 2 months; before usage, add β-mercaptoethanol to a final concentration of 5 nM and heat up to 37°C.
