REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-mouse CD3e (FITC/APC; 17A2, working dilution for FACS: 1:250 )	Invitrogen	11-0032-82/17-0032-82
Anti-mouse CD45 (eFluor450; 30-F11, working dilution for FACS: 1:250)	Invitrogen	18-0451-82
Anti-mouse CD11b (PerCP-Cy5.5/PE-Cy7; M1/70; working dilution for FACS: 1:250)	Invitrogen	45-0112-82/25-0112-82
Anti-mouse CD4 (PerCP-Cy5.5; RM4-5; working dilution for FACS: 1:250)	Invitrogen	45-0042-82
Anti-mouse F4/80 (FITC/APC eF780; BM8; working dilution for FACS: 1:250)	Invitrogen	11-4801-82 / 47-4801-82
Anti-mouse MHCII (PE; NIMR4; working dilution for FACS: 1:250)	Invitrogen	12-5322-81
Anti-mouse CD178 (APC; MFL3; working dilution for FACS: 1:250)	Invitrogen	17-5911-82
Anti-mouse CD16/32 (Fc Blocker; 93; working dilution for FACS: 1:1000)	Invitrogen	14-0161-82
Anti-mouse CD28 Monoclonal Antibody (funct. grade / purified; working concentration 0.1 μg/μL)	Invitrogen	16-0281-82
Chemicals, peptides, and recombinant proteins
DMEM+GlutaMAX(4,5 g/I D-Gluc./ Pyruvate)	GIBCO	31966-021
Gentamicin (50 mg/mL)	GIBCO	15750-045
RPMI1640 (L-Glutamine / 25mM HEPES)	GIBCO	52400-025
Penicilin / Streptomycin	GIBCO	15140-122
Fetal calf serum (FCS)	GIBCO	105000-064
Recombinant Murine M-CSF	PeproTech	315-02
2-Mercaptoethanol	GIBCO	31350010
LPS from E.coli	Adipogen	IAX-100-013-M001
HBSS	GIBCO	14175-053
FACS staining buffer	Thermo Fisher Scientific	00-4222-26
Cresyl Violet acetate (working concentration 0.5 %)	Sigma-Aldrich	C5042-10G
Critical commercial assays
T cell enrichment MagniSort	Thermo Fisher Scientific	8804-6820-74
Experimental models: Organisms/strains
C57BL6/J (male or female; 6–12 weeks old)	Charles River Laboratories	000664
C57BL/6-Tg(CAG-EGFP)1Osb/J (male or female; >8 weeks old)	The Jackson Laboratory	003291
Experimental models: Cell lines
NCTC clone 929 [L cell, L-929]	ATCC	CCL-1
Software and algorithms
BD FACSuite	Beckton Dickinson	N/A
Microsoft Excel	Microsoft Corporation	N/A
FlowJo v.10.6	Treestar Inc.	N/A
GraphPad Prism 6	GraphPad Software, Inc.	N/A
Creative Suite 6	Adobe	N/A
ImageJ Software	NIH, US	N/A
Other
40 μm cell strainer	FALCON	352340
50 mL Conical Centrifuge Tubes	FALCON	352070
4 mL Round-Bottom Polystyrene Test Tubes	FALCON	352052
Cell culture dish	Thermo Fisher Scientific	168381
Cell Scraper	FALCON	353086
Counting slides	Bio-Rad Laboratories	145-0011
Forceps	Fine Science Tools	11253-25
Scissors	Fine Science Tools	14090-09
Automated Cell counter	Bio-Rad Laboratories	TC20
Neubauer counting chamber	CLS Medizintechnik	A51005
96-Well, Cell Culture-Treated, U-Bottom Microplate	Thermo Fisher Scientific	3799
Injekt F Syringe	B. Braun	9166017
Cell culture dish	CELLSTAR	639960
5mL FACS tube	FALCON	352052