Table 2.

Thermodynamic parameters of binding between sdAbs and N‐proteins.

sdAb	Construct of N‐protein	No. of sites (N)	Kd (µm)	ΔH (kcal·mol−1)	TΔS (kcal·mol−1)	ΔG (kcal·mol−1)
sdAb‐N1	FLN	‐	No binding	‐	‐	‐
sdAb‐N2	FLN	0.457	1.75 ± 0.179	4.44 ± 0.146	−12.3	−7.86
	NLC	0.530	2.24 ± 0.307	4.40 ± 0.206	−12.1	−7.71
	NC	0.495	1.77 ± 0.217	3.66 ± 0.147	−11.5	−7.85
	CTD	0.292	2.38 ± 0.243	5.85 ± 0.141	−13.5	−7.67
	NTD	‐	No binding	‐	‐	‐
sdAb‐N3	FLN	0.066	4.37 ± 1.68	−80 ± 99.8	72.7	−7.31
	NLC	0.157	1.09 ± 0.485	−2.90 ± 0.558	−5.18	−8.14
	CTD	‐	No binding	‐	‐	‐
	NTD	‐	No binding	‐	‐	‐
sdAb‐N4	FLN	‐	No binding	‐	‐	‐
sdAb‐N5	FLN	0.697	3.97 ± 2.42	−11.7 ± 3.22	4.3	−7.37
	NLC	‐	No binding	‐	‐	‐
sdAb‐N6	FLN	0.416	3.53 ± 0.50	−17.8 ± 1.26	10.4	−7.44
	NLC	‐	No binding	‐	‐	‐