FIGURE 14.

Smartphone lateral flow point‐of‐care test for Ebola virus IgG detection. (a) Lateral flow strip illustration: serum applied onto the sample pad migrates through the analytical area, and subsequently forms complexes between the labeled gold nanoparticles (AuNPs) and the target analytes. Specifically targeted IgG serum antibodies against single or multiple recombinant Ebola viral proteins then bind to preprinted test lines, forming a visual red‐purple line. A control line is used to validate the assay function for the detection of antihuman antibody‐gold nanoparticle conjugates. Assay results appear after 15 min. (b) Illustration of the smartphone application (app) interface login window to record patient details; following submission, the analysis window opens; once the red box is aligned between the test and control lines, a tap on the screen provides the strips' analysis; result analysis window, which presents the relative intensity of the test line and determines whether the result is positive or negative based on an evaluated cutoff threshold. The window also provides a summary of patient details and a description of the test taken. Reprinted with permission from Brangel et al. (2018). American Chemical Society