Figure 4.

Inhibition of syncytium formation by the antibodies. a) Representative images of SARS‐CoV‐2 and SARS‐CoV spike‐mediated syncytium formation with hACE2 expressing cells 48 h after co‐culture. b,d) Syncytium number calculation and inhibition rates when treated with 10 nm of hACE2 competitive antibodies are shown. S‐RBD was used as the positive control (n = 5). c,e) Syncytium number and inhibition after treatment with antibodies and S‐RBD at different concentrations are shown (n = 5). f) The inhibition rates at 10 and 1 nm are summarized. Bars = 400 µm. Error bars indicate SD, ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001, determined by Student's t‐test in GraphPad Prism software.