FIGURE 9.

Measurement of cytokine levels secreted by splenocytes of immunised mice. BALB/c mice were immunised two times biweekly, and 2‐week post‐immunisation, spleens were harvested. Splenocytes were isolated and stimulated ex vivo in the presence of the purified inactivated virus 60 h. Cytokines released into culture media were determined by ELISA kit. Data are mean ± SD. The levels of statistical significance for differences between test groups were determined using ANOVA followed by Tukey’s post hoc test. *Statistical significance (P < 0.05)