FIGURE 12.

Measurement of Gzm B activity in mice groups that received different vaccine preparations. Immunisations were carried out two times over 2 weeks with 100 μL intramuscular injection of different vaccine preparations. The control groups received alum or PBS. At 2 weeks after the last immunisation, spleens were isolated from immunised mice and Gzm B activity was measured. Data are reported as mean ± SD. The levels of statistical significance for differences between test groups were determined using one‐way ANOVA followed by Tukey’s post hoc test. (a) Statistical significance (P < 0.0001) in comparison with groups that received 3 and 5 μg antigen alone. (b) Statistical significance (P < 0.0001) between groups that received 3 μg antigens with the groups immunised with 5 μg antigens