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. 2021 Oct 25;40(24):e108944. doi: 10.15252/embj.2021108944

Figure 2. SARS‐CoV‐2 variant infection increases formation of syncytia in U2OS‐ACE2 and Vero GFP‐split cells.

Figure 2

  1. U2OS‐ACE2 or Vero cells expressing either GFP 1–10 or GFP 11 (1:1 ratio) were infected 24 h after plating and imaged 20 h (U2OS‐ACE2) or 48 h (Vero) post‐infection.
  2. Left Panel: Fusion was quantified by GFP area/ number of nuclei and normalized to D614G for U2OS‐ACE2 20 h post‐infection at MOI 0.001. Right Panel: Representative images of U2OS‐ACE2 20 h post‐infection, GFP‐Split (green), and Hoechst (blue). Top and bottom are the same images with and without Hoechst channel.
  3. Left Panel: Quantified fusion of Vero cells infected at MOI 0.01. Right Panel: Representative images of Vero cells 48 h post‐infection, GFP‐Split (green), and Hoechst (blue).

Data information: Scale bars: 200 µm. Data are mean ± SD of eight independent experiments. Statistical analysis: one‐way ANOVA compared with D614G reference, ns: non‐significant, **P < 0.01, ****P < 0.0001.