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. 2021 Oct 25;40(24):e108944. doi: 10.15252/embj.2021108944

Figure EV4. SARS‐CoV‐2 variant S protein‐associated mutations are expressed equally at the cell surface.

Figure EV4

293T cells were transfected with S proteins with each of the variant‐associated mutations for 18 h and stained with human pan‐coronavirus mAb10 without permeabilization.
  1. Representative FAC plots of percent of cells expressing each mutant S protein at the surface.
  2. Quantification of percent of cells expressing each S protein at the surface.
  3. Quantification of median florescent intensity (MFI) of the mutant S protein at the cell surface.
  4. Representative histograms of MFI of each mutant S protein.
  5. Representative images of Vero GFP‐split cells 20 h after transfection with each Alpha variant‐associated mutant S protein, GFP‐Split (Green). Scale bars: 200 µm.
  6. Representative images of Vero GFP‐split cells 20 h after transfection with each Beta variant‐associated mutant S protein. Scale bars: 200 µm.

Data information: Data are mean ± SD of at least three independent experiments. Statistical analysis: one‐way ANOVA compared with D614G reference, ns: non‐significant.