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. 2021 Oct 25;40(24):e108944. doi: 10.15252/embj.2021108944

Figure 5. ACE2 and monoclonal antibody binding to S proteins with Alpha and Beta associated mutations.

Figure 5

  1. 293T cells were transfected S proteins with each variant‐associated mutation for 24 h and stained with biotinylated ACE2 and fluorescent streptavidin before analysis by flow cytometry.
  2. Left Panel: EC50 values (concentration of ACE2 needed for 50% binding) for Alpha and associated mutations. Color code corresponds to location on S protein functional domains and lower EC50 values signify higher affinity to ACE2 binding. Right Panel: EC50 values for Beta and associated mutations. Data set for N501Y and D614G reference mutations are duplicated between left and right panels as mutations are common to both variants.
  3. S protein transfected 293T cells were stained with human monoclonal antibodies targeting the S2 (mAb10), RBD (mAb48 and mAb98), and the NTD (mAb71). Cells were analyzed by flow cytometry. The percentage of positive cells is indicated.

Data information: Data are mean of at least three independent experiments. Statistical analysis: one‐way ANOVA compared with D614G reference, ns: non‐significant, *P < 0. 05, **P < 0.01, ****P < 0.0001.