Determination of primer specificity for M. ulcerans by 2426 PCR of 23 other species of mycobacteria as described in Materials and Methods. Lane 1, mycobacterial DNA pool 1; lane 2, pool 2; lane 3, pool 3; lane 4, pool 4; lane 5, pool 5; lane 6, M. ulcerans Malaysian strain 186510; lane 7, 100-bp ladder size marker; lane 8, no-template control.