Figure EV3. Loss‐of‐function effect of Tet2 and Tet3 on cell proliferation, survival, and differentiation.

• A
  mRNA expression of Tet1, Tet2, and Tet3 in BMMs stimulated with RANKL for 0 and 2 days (RNA‐seq analysis; n = 3 biological replicates). Data denote mean ± s.e.m.
• B
  mRNA expression of Tet2 and Tet3 in control‐ and Tet2_Rank ^–/–; Tet3a_Rank ^+/– ‐derived BMMs cultured in the absence and presence of RANKL for 2 days (quantitative RT–PCR analysis; n = 3 biological replicates). Data denote mean ± s.e.m.
• C
  Protein expression in control‐ and Tet2_Rank ^–/–; Tet3a_Rank ^+/– ‐derived BMMs cultured in the absence and presence of RANKL for 2 days. Arrows and arrowhead indicate Tet2 and Tet3 proteins, respectively.
• D, E
  Percentage of BrdU‐labeled CD11b⁺ BMMs (D) and Annexin V⁺ CD11b⁺ BMMs (E) derived from control‐ and Tet2_Rank ^–/–; Tet3a_Rank ^+/– mice. Data denote mean ± s.e.m. NS, not significant (n = 5 (D) and n = 3 (E) biological replicates; t‐test).