Skip to main content
. 2021 Oct 18;22(12):e53035. doi: 10.15252/embr.202153035

Figure EV4. Effect of hypoxia on the expression of Tet2 and Tet3, and osteoclast differentiation.

Figure EV4

  • A
    mRNA expression of Tet2 and Tet3 in BMMs stimulated with RANKL for 2 days under 5% and 2% oxygen. Data denote mean ± s.e.m. **P < 0.01; NS, not significant (n = 4 biological replicates; t‐test).
  • B
    Protein expression of Tet1, Tet2, and Tet3 in BMMs stimulated with RANKL for 2 days under 5% and 2% oxygen. Arrows and arrowhead indicate Tet2 and Tet3 proteins, respectively.
  • C
    Effect of hypoxia on osteoclastogenesis. TRAP‐stained cells (left panel) and the number of TRAP‐positive cells with more than three nuclei (right). Scale bar, 100 μm. Data denote mean ± s.e.m. **P < 0.01 (n = 6 biological replicates; ANOVA).