FIG. 2.

ΔTrkA transforms RIE-1 cells. RIE-1 cell lines were made that stably express empty vector, TrkA, ΔTrkA, and H-Ras61L. (A) Expression of Trk proteins was analyzed by Western blotting with anti-Trk antibodies. The position of TrkA and ΔTrkA proteins are indicated, and the molecular masses of standards are indicated at left. (B) ΔTrkA morphologically transforms RIE-1 cells, while wild-type TrkA does not. The highly refractile and morphologically transformed ΔTrkA cells are essentially indistinguishable from those caused by oncogenic H-Ras61L.