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ΔTrkA is constitutively tyrosine phosphorylated in RIE-1 cells and 32D myeloid progenitor cells. RIE-1 cells (A) and 32D cells (B) stably expressing empty vector, TrkA, and ΔTrkA were analyzed by Western blotting with antibodies that recognize phosphorylated tyrosine 490 of TrkA (PY490) (top panels). The blots were stripped and reprobed with anti-Trk antibodies (bottom panels).
