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NGF does not increase the tyrosine phosphorylation of ΔTrkA. RIE cells expressing either vector, TrkA, or ΔTrkA were treated with NGF for the times indicated (in minutes). Total cell lysates were collected and analyzed by Western blotting with anti-PY490, which specifically recognizes phosphorylation of tyrosine 490 of TrkA (top panel). The blot was stripped and western blotted with anti-Trk antibodies (bottom panel).
