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. Author manuscript; available in PMC: 2021 Dec 6.
Published in final edited form as: J Am Chem Soc. 2020 Dec 8;142(51):21260–21266. doi: 10.1021/jacs.0c09926

Figure 4.

Figure 4.

Functionalization of EGFP via the photoredox methionine bioconjugation protocol, (a) EGFP was initially labeled with 17, and then azides containing biological motifs were appended to the modified EGFP via CuAAC. (b) Azides bearing a biotin tag and a nonaarginine peptide, (c) After functionalization of EGFP via the photoredox methionine bioconjugation protocol, the product retained 95% of the fluorescence relative to wild-type EGFP (n = 3, mean ± SD). (d) Western blot images of wild type EGFP and modified EGFPs. (e) Confocal microscopy images of SJSA-1 cells treated with EGFP, EGFP–alkyne, and EGFP–(Arg)9. Scale bars = 20 μm.