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. Author manuscript; available in PMC: 2021 Dec 6.
Published in final edited form as: Scand J Gastroenterol. 2021 May 3;56(7):791–805. doi: 10.1080/00365521.2021.1917650

Figure 6.

Figure 6.

TFF2 has weak affinity interactions with LINGO3. (A) Approach for incubation of soluble ligand with transfected HEK cells followed by flow cytometry to identify doubly fluorescent cells. Percent of doubly fluorescent cells identified in the upper right quadrant for LINGO3-GFP transfectants incubated with (B) PBS (C) IgG-Alexa Fluor 647 or (E) TFF2- Alexa Fluor 647. (D) LINGO2-GFP transfected HEK cells incubated with TFF2-Alexa Fluor 647. (F) CXCR4-GFP transfectants incubated with (F) TFF2-Alexa Fluor 647 or (G) SDF1- Alexa Fluor 647, a known high affinity binding partner. (H) Coimmunoprecipitation of Flag-LINGO3 and TFF2-Fc in transfected CHO cells. (I) Control TFF2-Fc and Fc without co-immunoprecipitation migrate at 50 kD and 40 kD.