Figure 4. LncRNA CRNDE induces cisplatin resistance in GC cells.

• A–D
  Exosomes were isolated from the media of BMDMs (BMDMs‐exo), BMDM‐polarized M2 macrophages (M2‐exo), BMDM‐polarized M2 macrophages transfected with si‐CRNDE (M2‐si‐CRNDE‐exo), and BMDM‐polarized M2 macrophages transfected with the negative control of si‐CRNDE (M2‐si‐control‐exo). Then, MFC cells were treated with indicated exosomes and cisplatin (CDDP; 1 μg/ml) for 48 h. (A) LncRNA CRNDE expression in indicated exosomes was detected by qRT–PCR. (B) The survival rate of MFC cells was measured by cell counting kit‐8 (CCK‐8) assay. (C) Representative images of colony formation assay performed on MFC cells. (D) Cell apoptosis was measured by flow cytometry.
• E–H
  Exosomes were isolated from the medium of monocytes (monocytes‐exo), monocyte‐polarized M2 macrophages (M2‐exo), monocyte‐polarized M2 macrophages transfected with si‐CRNDE (M2‐si‐CRNDE‐exo), and monocyte‐polarized M2 macrophages transfected with si‐control (M2‐si‐control‐exo). Then, SGC7901 cells were treated with indicated exosomes and CDDP (1 μg/ml). (E) LncRNA CRNDE expression, (F) cell survival rate, (G) cell proliferation, and (H) cell apoptosis were measured.

Data information: (A, D, E, and H): The results shown are from three biological replicates. (B and F): The results shown are from seven biological replicates. Data are expressed as mean ± SD. (A, B, D, E, F, and H): one‐way ANOVA. **P < 0.01.