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A
Double fluorescence in situ hybridization images indicating co‐labeling of Slc4a4 (green) and GLAST (red). Scale bar: 100 μm.
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B
Schematics showing induction of temporally controlled astrocyte‐specific conditional null alleles.
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C
In situ hybridization confirms deletion of Slc4a4 in the cortex. GFAP immunostaining intensity was decreased significantly in Slc4a4 icKO at P12 whereas Sox9+ cell number remained unchanged. Scale bar: 100 μm.
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D
Co‐immunoprecipitation revealing that Slc4a4 associates with Daam2 in vivo.
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E
Schematics showing induction of temporally controlled astrocyte‐specific conditional null alleles.
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F–J
Representative images of GFAP immunostaining (F) and quantification (G–J) in controls, Daam2 icKO, Slc4a4 icKO, and double icKO 6 weeks after tamoxifen injection. Scale bar: 20 μm. Data are presented as mean ± SEM. n = 10–24 images, N = 3–4 mice per genotype. Scale bar: 20 μm. Two‐way ANOVA is used for statistics. [*] and [#] indicates comparison with control and double icKO, respectively. **P < 0.01, ***P < 0.001, ##
P < 0.01.
