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. 2021 Sep 30;22(12):e52727. doi: 10.15252/embr.202152727

Figure 9. IF1 is beneficial for ATP production even under OXPHOS conditions.

Figure 9

  1. Mitochondrial ATP production rates in HeLa cells the presence and absence of IF1. Oxygen consumption rates (OCR) and extracellular acidification rates (ECAR) were determined with an automatic flux analyzer (Seahorse/Agilent). After monitoring basal respiration, oligomycin (1 µM), and rotenone plus antimycin A (Rot; 0.5 µM; AA, 0.5 µM) were added sequentially to determine glycolytic and mitochondrial ATP production rates. ˜12 technical replicates per measurement, N = 4 independent assays.
  2. Cytosolic ATP production rates. Each data point represents the mean ATP production rate of one well (˜12 technical replicates per measurement, N = 4 independent assays.).
  3. Determination of mitochondrial ATP levels with the fluorogenic dye ATP‐red™ (5 µM, prestaining for 15 min). To normalize on mitochondrial mass, mitochondria were stained with MitoTracker™ Green (MTG, 100 nM for 30 min). Two technical replicates from same clones.

Data information: (A–C) Statistics: One‐way ANOVA with post hoc Scheffé test. The error bars denote SD; the boxes represent the 25th to 75th percentiles. Whiskers: outliners include coefficient 1.5. The vertical lines in the boxes represent the median values, whereas the square symbols in the boxes denote the respective mean values. The minimum and maximum values are denoted by x.***P ≤ 0.001; **P ≤ 0.01; n.s.: non‐significant.