Total mononuclear and CD34+ cell count are lower in SCD BM compared to non-SCD BM. (A) Comparison of SCD BM (n = 10) versus non-SCD BM (n = 5) total mononuclear cell count combing all data, or comparing various methods of processing for all samples including collection in heparin, ACDA, or processing immediately or 1 day after collection. (B) For all data and each processing condition, CD34+ cell count is lower in SCD BM (n = 10) compared to non-SCD marrow (n = 5). *P < 0·05. ACDA, acid citrate dextrose-A; BM, bone marrow; SCD, sickle cell disease.