Fig 3.

Imagestream data show lower purity in CD34⁺ cells in subjects with SCD. Image flow cytometry performed on CD34⁺-selected HSCs collected in two different anticoagulants (heparin and ACDA) and processed immediately after collection demonstrated a significantly lower proportion of CD34^bright HSCs in SCD marrow (n = 10) compared to non-SCD marrow (n = 5). (A) Greater than 50% of SCD HSCs are characterized as CD34^dim. (B) ImageStream histogram plot displays two populations of CD34⁺ antibody intensity corresponding to CD34^bright and CD34^dim populations. ***P < 0·001. ACDA, acid citrate dextrose-A; APC, allophycocyanin; BM, bone marrow; SCD, sickle cell disease.