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. 2021 Dec 1;14:6397–6407. doi: 10.2147/JIR.S339133

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© 2021 Sun et al.

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The overexpression of circFoxo3 attenuates OGD-induced cardiomyocyte injury in vitro. (A) The back-spliced and canonical forms of Foxo3 expression was detected by agarose gel electrophoresis assays and PCR in H9c2 cells in the presence or absence of RNase R supplementation. (B–F) H9c2 cells were treated with OGD along with transfection of circFoxo3 overexpressing vectors (OE circ) or empty vectors (NC) for 48 hours. (B) Relative level of circFoxo3 RNA was detected by qRT-PCR.²⁵ Cell viability was determined by CCK-8 assay. (D) Cell apoptosis was determined by flow cytometry. (E) The levels of IL-6 and IL-10 in culture medium were checked by ELISA assay. **p < 0.01.