Table.1.
Reading methods results of MIC and susceptibility from broth microdilution assays for Nocardia spp. isolates
| Isolates | Drugs | Visual Readings | OD Readings | ||||
|---|---|---|---|---|---|---|---|
| Gold Standard | With Resazurin | ||||||
| Modal MIC (range) | aSusc. (%) | Modal MIC (range) | aSusc. (%) | Modal MIC (range) | aSusc. (%) | ||
| N. brasiliensis-83 | AMK | 0.5 (0.5–2.0) | 100.0 | 0.5 (0.5–1.0) | 100.0 | 1.0 (0.5–1.0) | 100.0 |
| CIP | 0.12 (0.12) | 100.0 | 0.12 (0.12) | 100.0 | 0.12 (0.12) | 100.0 | |
| MIN | 8.0 (4.0–16.0) | 0.0 | 4.0 (4.0–8.0) | 0.0 | 4.0 (2.0–8.0) | 0.0 | |
| TMP–SMX | 0.5 (0.12–2.0) | 100.0 | 4.0 (0.25–8.0) | 37.5 | 0.25 (0.12–0.5) | 100.0 | |
| N. brasiliensis-95 | AMK | 0.5 (0.5–1.0) | 100.0 | 2.0 (0.5–2.0) | 100.0 | 1.0 (0.5–1.0) | 100.0 |
| CIP | 4.0 (4.0) | 0.0 | 4.0 (4.0) | 0.0 | 4.0 (2.0–4.0) | 0.0 | |
| MIN | 1.0 (0.25–1.0) | 100.0 | 0.5 (0.25–2) | 90.0 | 1.0 (0.5–1.0) | 100.0 | |
| TMP–SMX | 0.12 (< 0.12–0.5) | 100.0 | 0.12 (0.12–2) | 100.0 | 0.12 (< 0.12–2.0) | 100.0 | |
| N. brasiliensis-129 | AMK | 1.0 (0.5–1.0) | 100.0 | 2.0 (2.0–4.0) | 100.0 | 1.0 (1.0–2.0) | 100.0 |
| CIP | 4.0 (4.0) | 0.0 | 4.0 (4.0) | 0.0 | 4.0 (2.0–4.0) | 0.0 | |
| MIN | 0.5 (0.25–1.0) | 100.0 | 1.0 (0.25–1.0) | 100.0 | 0.5 (0.25–1.0) | 100.0 | |
| TMP–SMX | 0.25 (0.12–0.5) | 100.0 | 0.25 (0.25–1.0) | 100.0 | 0.25 (0.25–1.0) | 100.0 | |
| N. brasiliensis-514 | AMK | 0.5 (0.5–1.0) | 100.0 | 0.5 (0.5–1.0) | 100.0 | 0.5 (0.5–4.0) | 100.0 |
| CIP | 0.12 (0.12) | 100.0 | 0.12 (0.12) | 100.0 | 0.12 (0.12) | 100.0 | |
| MIN | 4.0 (4.0–16.0) | 0.0 | 8.0 (4.0–8.0) | 0.0 | 4.0 (2.0–8.0) | 0.0 | |
| TMP–SMX | 2.0 (0.25–8.0) | 62.5 | 4.0 (2.0–32.0) | 14.3 | 0.12 (0.12–8.0) | 87.5 | |
| N. brasiliensis-519 | AMK | 0.5 (0.5) | 100.0 | 0.5 (0.5–1.0) | 100.0 | 1.0 (0.5–1.0) | 100.0 |
| CIP | 2.0 (1.0–2.0) | 50.0 | 1.0 (1.0–2.0) | 71.4 | 2.0 (2.0) | 0.0 | |
| MIN | 0.5 (0.06–1.0) | 100.0 | 1.0 (0.12–2.0) | 80.0 | 0.25 (0.06–0.25) | 100.0 | |
| TMP–SMX | 0.12 (0.12–0.25) | 100.0 | 0.12 (0.12–1.0) | 100.0 | 0.12 (0.12–0.25) | 100.0 | |
| N. brasiliensis-608 | AMK | 2.0 (0.5–2.0) | 100.0 | 2.0 (0.5–2.0) | 100.0 | 2.0 (2.0) | 100.0 |
| CIP | 4.0 (0.12–32.0) | 22.2 | 4.0 (2.0–4.0) | 0.0 | 4.0 (0.12–4.0) | 33.3 | |
| MIN | 0.06 (0.06–0.12) | 100.0 | 0.06 (0.06–0.5) | 100.0 | 0.06 (0.06–0.12) | 100.0 | |
| TMP–SMX | 1.0 (0.25–1.0) | 100.0 | 0.5 (0.12–1.0) | 100.0 | 0.25 (0.12–025) | 100.0 | |
| N. brasiliensis-775 | AMK | 0.5 (0.5–1.0) | 100.0 | 0.5 (0.5–1.0) | 100.0 | 1.0 (0.5–1.0) | 100.0 |
| CIP | 4.0 (4.0) | 0.0 | 4.0 (4.0) | 0.0 | 4.0 (4.0) | 0.0 | |
| MIN | 0.06 (0.06–0.5) | 100.0 | 1.0 (0.06–1.0) | 100.0 | 0.06 (0.06–0.25) | 100.0 | |
| TMP–SMX | 1.0 (0.12–1.0) | 100.0 | 1.0 (0.25–4.0) | 66.7 | 0.25 (0.25–1.0) | 100.0 | |
| N. brasiliensis-865 | AMK | 1.0 (0.5–2.0) | 100.0 | 2.0 (1.0–2.0) | 100.0 | 0.5 (0.5–2.0) | 100.0 |
| CIP | 4.0 (0.25–8.0) | 11.1 | 4.0 (2.0–4.0) | 0.0 | 4.0 (2.0–4.0) | 0.0 | |
| MIN | 0.5 (0.5–1.0) | 100.0 | 0.12 (0.06–0.12) | 100.0 | 1.0 (0.5–1.0) | 100.0 | |
| TMP–SMX | 0.12 (0.12–0.25) | 100.0 | 0.12 (0.12) | 100.0 | 0.12 (0.12–0.25) | 100.0 | |
| N. farcinica-935 | AMK | 0.5 (0.5–1.0) | 100.0 | 1.0 (0.5–2.0) | 100.0 | 0.5 (0.5–1.0) | 100.0 |
| CIP | 64.0 (32.0–64.0) | 0.0 | 64.0 (32.0–64.0) | 0.0 | 16.0 (16.0–32.0) | 0.0 | |
| MIN | 0.12 (0.06–1.0) | 100.0 | 0.06 (0.06–2.0) | 91.6 | 0.06 (0.06–0.12) | 100.0 | |
| TMP–SMX | 1.0 (0.25–4.0) | 83.3 | 4.0 (0.25–8.0) | 41.6 | 0.25 (0.25–1.0) | 100.0 | |
| N. otitidiscaviarum-779 | AMK | 0.5 (0.5–1.0) | 100.0 | 0.5 (0.5–1.0) | 100.0 | 0.5 (0.5–1.0) | 100.0 |
| CIP | 4.0 (4.0–64.0) | 0.0 | 8.0 (8.0–16.0) | 0.0 | 8.0 (1.0–4.0) | 33.3 | |
| MIN | 0.06 (0.06–0.25) | 100.0 | 0.06 (0.06–1.0) | 100.0 | 0.06 (0.06–0.25) | 100.0 | |
| TMP–SMX | 0.5 (0.25–0.5) | 100.0 | 0.5 (0.25–8.0) | 62.5 | 0.5 (0.12–1.0) | 100.0 | |
| N. asteroides-788 | AMK | 0.5 (0.5–1.0) | 100.0 | 1.0 (0.5–2.0) | 100.0 | 0.5 (0.5) | 100.0 |
| CIP | 0.5 (0.25–2.0) | 100.0 | 0.5 (0.5–2.0) | 100.0 | 0.25 (0.25–1.0) | 100.0 | |
| MIN | 4.0 (2.0–4.0) | 0.0 | 4.0 (4.0–8.0) | 0.0 | 2.0 (2.0–4.0) | 0.0 | |
| TMP–SMX | 2.0 (0.25–4.0) | 75.0 | 2.0 (0.25–32.0) | 50 | 0.25 (0.12–0.5) | 100.0 | |
| N. otitidiscaviarum-937 | AMK | 0.5 (0.5–1.0) | 100.0 | 0.5–1.0 (1.0) | 100.0 | 0.5 (0.5–1.0) | 100.0 |
| CIP | 2.0 (1.0–2.0) | 22.2 | 2.0 (2.0–4.0) | 0.0 | 2.0 (0.25 –2.0) | 28.6 | |
| MIN | 0.06 (0.06–0.12) | 100.0 | 0.06 (0.06–1.0) | 100.0 | 0.06 (0.06–0.25) | 100.0 | |
| TMP–SMX | 1.0 (0.25–1.0) | 100.0 | 1.0 (0.25–2.0) | 100.0 | 0.25 (0.12–0.5) | 100.0 | |
| N. beijingensis-1180 | AMK | 0.5 (0.5–1.0) | 100.0 | 0.5 (0.5–1.0) | 100.0 | 0.5 (0.5) | 100.0 |
| CIP | 2.0 (2.0–4.0) | 0.0 | 4.0 (2.0–4.0) | 0.0 | 2.0 (2.0–8.0) | 0.0 | |
| MIN | 1.0 (0.5–2.0) | 60.0 | 1.0 (1.0–2.0) | 90.0 | 2.0 (1.0–4.0) | 30.0 | |
| TMP–SMX | 0.12 (0.12–0.25) | 100.0 | 0.12 (0.12–0.25) | 100.0 | 0.12 (0.12–1.0) | 100.0 | |
| N. beijingensis-1181 | AMK | 0.5 (0.5–1.0) | 100.0 | 0.5 (0.5–1.0) | 100.0 | 0.5 (0.5–1.0) | 100.0 |
| CIP | 4.0 (1.0–4.0) | 9.1 | 4.0 (0.12–8.0) | 20.0 | 4.0 (2.0–4.0) | 0.0 | |
| MIN | 1.0 (0.5–2.0) | 60.0 | 1.0 (0.5–2.0) | 66.6 | 2.0 (1.0–4.0) | 20.0 | |
| TMP–SMX | 0.12 (0.12–0.25) | 100.0 | 0.12 (0.12–0.25) | 100.0 | 0.12 (0.12–0.5) | 100.0 | |
| N. farcinica-2901 | AMK | 1.0 (1.0–2.0) | 100.0 | 2.0 (2.0–4.0) | 100.0 | 1.0 (1.0) | 100.0 |
| CIP | 0.25 (0.25) | 100.0 | 0.5 (0.25–4.0) | 85.7 | 0.25 (0.25) | 100.0 | |
| MIN | 2.0 (2.0) | 0.0 | 2.0 (2.0) | 0.0 | 2.0 (1.0–2.0) | 50.0 | |
| TMP–SMX | 8.0 (4.0–8.0) | 0.0 | 16.0 (16.0) | 0.0 | 1.0 (0.5–1.0) | 100.0 | |
aPercentage of Susceptibility