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PCR primers designed according to the mutational hotspots within four Stephania species
| Mutational hotspots | PCR primers | Expected length |
|---|---|---|
| trnH-psbA | F:CGCCGTAGTAAATAGGAGA | 860 bp |
| R:TCATCAACCGYGCTAACCT | ||
| rps16 | F:RATACAATAAGCAAGCTC | 957 bp |
| R:TCCCRAAACAAGAAAACG | ||
| trnD-trnY | F:GTGCTCTGACCGATTGAACT | 525 bp |
| R:GGCAATATGTCTACGCTGGT | ||
| trnP | F:TAGGTAGGGATGACAGGA | 918 bp |
| R:GACCCGAACCATAGAGTA | ||
| ndhA | F:CRAATCCMAAATTAGACCA | 668 bp |
| R:GACGCTTAGGAACACCAA |
