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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Reproduction. 2021 Jan;161(1):73–88. doi: 10.1530/REP-19-0433

Figure 1. Cell culture timeline for BeWo cell NEFA-treatments.

Figure 1.

BeWo cells were plated and allowed to adhere overnight prior to NEFA-treatment initiation at T0H. At T24H 250 μM 8-Br-cAMP or a vehicle control was added to the cell culture plates to allocate cells to either CT or SCT cell type. All metabolic activity assays and cell collections were performed at T72H.