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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Reproduction. 2021 Jan;161(1):73–88. doi: 10.1530/REP-19-0433

Figure 3. Mitochondrial respiratory activity of BeWo cells following prolonged NEFA treatment.

Figure 3.

Oxygen Consumption Rate (OCR) of experimental media was measured under basal conditions and following subsequent injections of: Oligomycin (1.5 μg/mL); Dinitrophenol (50 μM); and Rotenone and Antimycin A (0.5 μM each) to calculate the (A) Basal Respiration, (B) Maximal Respiration, (C) Proton Leak, (D) Spare Respiratory Capacity, and (E) Coupling Efficiency in NEFA-treated BeWo CT and SCT cells (n=5/group). (A, B, C) Statistical analysis was performed on DNA-normalized OCR rates via Two-Way Randomized Block ANOVA, Sidak’s multiple comparisons Test; (D, E) percent basal OCR was log transformed and analyzed via Two-Way ANOVA). (A, B, C) Respiratory activity data is expressed as OCR normalized to Fsutotal DNA content and presented as percent CT untreated control; (D, E) data is represented as percent basal OCR for each condition. Different letters denote statistical significance (p<0.05, n = 5/group).