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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Reproduction. 2021 Jan;161(1):73–88. doi: 10.1530/REP-19-0433

Figure 6. Prolonged NEFA treatments did not affect protein expression of enzymes involved in mitochondrial uptake of pyruvate or long-chain fatty acid species.

Figure 6.

Relative protein abundance of (A) Pyruvate Dehydrogenase (PDH), (B) PDH-E1α (pSer232) (pPDH), (C) the pPDH/PDH ratio, (D) Pyruvate Dehydrogenase Kinase-1 (PDHK1) (E) Lactate Dehydrogenase (LDH), and (F) CPT1a was determined in NEFA-treated BeWo CT and SCT cells at 72H via immunoblotting. Data is presented as percent CT untreated control (n=5/group). Representative full length ponceau loading control images are available in Supplementary Figure 3.