Table 3:
Maximal activity rates of mitochondrial enzymes in NEFA-treated BeWo CT and SCT cells. Data are mean ± SEM of % untreated CT U/mg protein.
| Cell Type / Treatment | Citrate Synthase | ETC Complex I | ETC Complex II | Lactate Dehydrogenase | PDH-E1 subunit | Pyruvate Dehydrogenase | |
|---|---|---|---|---|---|---|---|
| CT | |||||||
| BSA Ctrl | 116.20 ± 22.37 | 120.7 ± 7.71 | 113.8 ± 13.71 | 163.20 ± 45.50 | 99.23 ± 6.37 | 105.04 ± 6.93a | |
| PA | 93.62 ± 15.00 | 98.00 ± 10.03 | 98.99 ± 19.80 | 109.80 ± 14.41 | 101.72 ± 3.83 | 86.78 ± 10.96b | |
| OA | 85.31 ± 14.22 | 100.00 ± 5.59 | 94.46 ± 17.77 | 104.90 ± 19.23 | 102.84 ± 16.80 | 112.01 ± 10.41a | |
| P/O | 90.34 ± 5.44 | 106.20 ± 0.32 | 93.67 ± 17.50 | 133.60 ± 31.71 | 118.96 ± 21.97 | 99.56 ± 7.37ab | |
| SCT | |||||||
| BSA Ctrl | 60.68 ± 6.01 | 191.50 ± 31.49a | 61.76 ± 13.75 | 79.25 ± 18.77 | 260.45 ± 41.81 | 84.66 ± 4.99 | |
| PA | 49.80 ± 4.60 | 183.10 ± 25.80a | 62.05 ± 15.57 | 79.54 ± 16.18 | 246.35 ± 20.16 | 88.20 ± 9.06 | |
| OA | 39.80 ± 4.74 | 129.2 ± 13.85ab | 51.88 ± 17.79 | 73.79 ± 17.63 | 222.28 ± 19.02 | 80.33 ± 3.51 | |
| P/O | 63.52 ± 4.14 | 103.60 ± 17.77b | 58.96 ± 8.20 | 78.59 ± 13.26 | 233.78 ± 57.00 | 81.68 ± 8.10 | |
| *Differentiation state difference | NS | * | * | NS | * | * | |
*
denotes a differentiation state dependent difference in enzyme activity between BeWo CT and SCT cells (n=5/group). Different letters denote significant differences between NEFA treatments within a differentiation state.