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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Nat Protoc. 2021 Jul 2;16(8):3802–3835. doi: 10.1038/s41596-021-00556-8

Fig. 8 |. Starting parameters for performing single-cell segmentation on the uploaded data by using the CODEX Segmenter.

Fig. 8 |

These include cell radius, max/min/relative cutoff, cell size cutoff factor, nuclear stain channel/cycle and if desired, membrane stain channel/cycle (in general, this parameter is not used, and thus the cycle is −1). These parameters will change for each experiment.