| Cycle | DAPI | Alexa448 | ATTO55O | Alexa647 | Volume of plate buffer (μl) | Total well volume (μl) |
|---|---|---|---|---|---|---|
| 1 | Hoechst | Blank | Blank | Blank | 250 | 250 |
| 2 | Hoechst | Marker 1 | Marker 2 | Marker 3 | 220 | 250 |
| 3 | Hoechst | Blank | Marker 4 | Marker 5 | 230 | 250 |
| 4 | Hoechst | Blank | Blank | Marker 6 | 240 | 250 |
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