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. Author manuscript; available in PMC: 2021 Dec 6.
Published in final edited form as: Cancer Res. 2021 Jan 13;81(5):1240–1251. doi: 10.1158/0008-5472.CAN-20-0118

Figure 1.

Figure 1.

N-RasG12D/+ confers HSC survival to exogenous stress in vitro. A, Experimental design for in vitro cytokine starvation. B, Representative flow cytometry plot for Annexin V and DAPI staining with quantitative representation (n = 4). Paired Student t test was used to calculate statistical significance. C, Survival, as measured by caspase activation, of control or NrasG12D SLAM HSCs cultured in cytokine-rich (SCF+TPO; 100 ng/mL) or starved SF-03 media (n = 6). Two-tailed unpaired Student t test was used to calculate statistical significance. WT, wild-type. D, Experimental design for in vitro radiation. E, Representative flow cytometry plot for Annexin V and DAPI staining following radiation with quantitative representation (n = 7 for 0 Gy, n = 3 for 3 Gy). Paired Student t test was used to calculate statistical significance. F, Survival of FACS-sorted control or NrasG12D HSCs, as measured by caspase activation, after exposure to 300 cGy of in vitro γ-radiation (n = 3). Two-tailed unpaired Student t test was used to calculate statistical significance. Data represent mean ± SD; n, number of mice.