Figure 3.

The four zebrafish gill arches display the same lymphoid organization. Representative deconvolved confocal images of an adult zebrafish branchial cavity showing the 4 gills arches with transversal orientation (A). The plane of section is illustrated by the scheme at the bottom left. The images were acquired from 30 μm whole-body cryosections stained with phalloidin (green) and DAPI (blue) and where T/NK cells were labeled with anti-ZAP70 antibody (red hot). Each gill arch (Ga1-Ga4) possesses a GIALT segmented in five sub-regions (1-5) (ILT, interlamellar region-lamellae-efferent aspect of filaments, interbranchial septum, gill arch, T/NK cell clusters at the base of filaments on each side of the gill arch). (B) Schematic representation of (A) displaying the 5 sub-regions of the GIALT. (A) The image is a maximum intensity projection of 15 µm. Annotations: Bc, Branchial cavity; Ga, Gill arch and S, Septum. Scale bar: 200 μm.