Skip to main content
. 2021 Sep 12;78(6):802–808. doi: 10.1097/FJC.0000000000001123

FIGURE 3.

FIGURE 3.

Overexpression of MFACR decreased the expression levels of miR-125b through methylation in AC16 cells. To study the effects of crosstalk between MFACR and miR-125b, AC16 cells were transfected with either MFACR expression vector or miR-125b mimic, followed by checking the overexpression of MFACR and miR-125b every 24 hours until 96 hours (A). The effects of overexpression of MFACR on the expression of miR-125b (B), and the effects of overexpression of miR-125b on the expression of MFACR (C) were analyzed by RT-qPCR. MSP was performed at 96 hours post-transfection to analyze the effects of the overexpression of MFACR on the methylation of miR-125b gene (D).M, methylation; U, un-methylation; *P < 0.05.