Skip to main content
. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Free Radic Biol Med. 2020 Dec 9;163:102–115. doi: 10.1016/j.freeradbiomed.2020.11.018

Figure 9.

Figure 9.

Protection against oxidative stress induced TJ disruption in HT-29 cells by Garcinone D requires AhR. (a,b) Representative immunoblots for ZO-1 and occludin. Cells were either untreated or treated with 5 μM CH-223191 (a potent and selective AhR antagonist) and subsequently co-incubated with 12.5 μM Garcinone D and 50 μM tBHP. Cell lysates were collected and immunoblotted with ZO-1 and anti-occludin antibodies; relative protein densities normalized to β-actin. Data are expressed as mean values ± SD from triplicate experiments. Bars bearing * (P ≤ 0.05) and ** (P ≤ 0.01) were significantly different by one-way ANOVA followed by Tukey-HSD test vs control.