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. 2021 Dec;31(12):2290–2302. doi: 10.1101/gr.275752.121

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© 2021 Dallaire et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genome Research, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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Figure 5. — Isolation of 2′-O-methylated and Argonaute-loaded small RNA. (A) Schematic representation of the sRNA-seq analysis pipeline used in this study. (B,C) Plot of mean normalized counts against log₂ fold change (log₂FC) in sRNAs sequenced following enrichment using NaIO₄ oxidation (B) or TraPR column purification (C), compared to sRNAs sequenced following no treatment. Points represent individual sRNA loci. Significantly differentially expressed loci are shown in red. Mitochondria-derived sRNA loci are shown in blue. (D–F) Length distribution and first nucleotide bias of the sRNAs in untreated (D), NaIO₄-treated (E), and TraPR column-extracted (F) sRNA libraries. (G) Significantly differentially expressed sRNA loci. sRNAs enriched by NaIO₄ or column-purification were induced compared to expression in untreated samples, and sRNAs depleted by NaIO₄ or column-purification were down-regulated.