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. 2021 Nov 22;12:773656. doi: 10.3389/fpls.2021.773656

FIGURE 1.

FIGURE 1

Efficiency testing of clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) and guide RNA (gRNA) activity using electro-transfection in oil palm protoplasts. (A) Schematic map of gRNA target site in phytoene desaturase (EgPDS) gene. (B) Oil palm protoplast before (left) and protoplast agglutination after (right) electro-transfection with Cas9/gRNA. (C) Cleavage efficiency of Cas9/gRNA in oil palm protoplast following electro-transfection. Data are average values ± SE from four independent experiments.