FIGURE 3.

Mutation events of targeted CRISPR/Cas9 mutagenesis of EgPDS gene in in vitro transgenic oil palm. Mutation events detected in the transgenic oil palms exhibiting the chimeric albino phenotypes using sequencing-based detection at the target sites of (A) gPDS4, (B) gPDS5, and (C) both gPDS4 and gPDS5 in the EgPDS gene and (D) the percentage frequency of occurrence for each type of mutation event. Protospacer adjacent motif sequence (PAM) is highlighted in yellow, InDels sequences of the target site are shown in red, and nucleotide substitution are marked in blue. g4, gPDS4; WT, wild-type; C, control; g5, gPDS5; Dm, double mutant; 1–5, plantlet number.