TABLE 2.

The efficiency of CRISPR/Cas9 and gRNA transformation targeting brassinosteroid-insensitive 1 (EgBRI1) gene in oil palm and the phenotypes of the transgenic plants.

Gene	Guide RNA	Bombarded immature embryo	Putative transformant	Regenerated T0 shoots	T0 with mutation/genotyped T0 plants	Mutation rate	Transformants with altered phenotype
Control	Cas9	200	22 (11%)	17 (77%)	0/5	0	0
	gBRI1-1	200	17 (8.5%)	13 (76.5%)	0/5	0	0
	gBRI1-2	200	10 (5%)	8 (80%)	0/5	0	0
EgBRI1	Cas9/gBRI1-1	200	24 (12%)	18 (75%)	10/17	58.82%	6 stunted (33%) 5 leaf necrosis (28%)
	Cas9/gBRI1-2	200	23 (11.5%)	20 (87%)	10/15	66.67%	5 stunted (25%) 10 leaf necrosis (50%)
	Cas9/gBRI1-1 + gBRI1-2	200	36 (18%)	30 (83%)	7/7	100%	30 stunted (100%)