Extended Data Table 1.
Statistics for 3D reconstruction and model refinement.
| States Codes | Hetero Pentamer (EMD-23913) (PDB 7MLY) |
Homo Tetramer (EMD-23911) (PDB 7MLV) |
Homo Trimer (EMD-23912) |
Homo Pentamer (EMD-23910) (PDB 7MLU) |
|---|---|---|---|---|
| Data collection and processing | ||||
| Microscope | Titan Krios | |||
| Camera Magnification | K3 BioQuantum 105,000 | |||
| Voltage (kV) | 300 | |||
| Defocus range (μm) | −1.2 to −2.2 | |||
| Exposure time (s) | 1.5 | |||
| Dose rate (e−/Å2/s) | 18.8 | |||
| Number of frames | 60 | |||
| Pixel size (Å) | 0.823 | |||
| Micrographs (no.) | 17,472 | |||
| Initial particles (no.) | 4,046,595 | |||
| Symmetry imposed | C1 | C1 | C1 | C5 |
| Final particles (no.) | 527,075 | 129,772 | 10,136 | 20,660 |
| Map resolution (Å) | 2.74 | 3.85 | 12.30 | 4.1 |
| FSC threshold | 0.143 | 0.143 | 0.143 | 0.143 |
| Refinement | ||||
| Initial model (PDB code) | - | 7MLY | - | 7MLY |
| Model resolution (Å) | 3.3 | 4.1 | - | 4.3 |
| FSC thrpqhold | 0.5 | 0.5 | - | 0.5 |
| Model composition | ||||
| Non-hydrogen atoms | 21640 | 15085 | - | 21891 |
| Protein atoms | 20410 | 14808 | - | 21821 |
| Ligand atoms | 1230 | 277 | - | 70 |
| B factors (Å2) | ||||
| Protein | 102.7 | 159.8 | - | 85.6 |
| Ligand | 124.1 | 321.6 | - | - |
| R.m.s. deviations | ||||
| Bond length (Å) | 0.004 | 0.004 | - | 0.004 |
| Bond angle (°) | 0.594 | 0.938 | - | 0.872 |
| Validation | ||||
| Favored (%) | 93.37 | 92.93 | - | 93.64 |
| Allowed (%) | 6.63 | 7.07 | - | 6.91 |
| Disallowed (%) | 0 | 0 | - | 0 |
| Poor rotamers | 0.04 | 0 | - | 0.21 |
| MolProbity score | 1.84 | 1.71 | - | 1.80 |
| Clash score | 7.44 | 4.92 | - | 6.81 |