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. 2021 Dec 6;16(12):e0260706. doi: 10.1371/journal.pone.0260706

Fig 2. Budesonide, formoterol or montelukast do not impact TEER, but budesonide attenuates small molecule flux.

Fig 2

16HBE cells were grown to confluency (over 800 ohm) and treated with vehicle or 0.5 μg/ml polyI:C and 0.1–1μM drug. A) TEER was monitored at 6, 24, 30, 48, and 72hrs post polyI:C addition. B) At 48hrs post polyI:C, 10 μg/ml 4kDa FITC-dextran was applied apically and the amount of FITC-dextran translocation to the basal chamber was quantified 2hrs later using a fluorescent plate reader. C) 16HBE cells were treated as in A and B, but lysed in RIPA buffer for protein analysis by Western Blot. Band intensity was quantified with ImageJ and values normalized to the loading control GAPDH. Data are mean ± standard deviation. One way ANOVA followed by unpaired Tukey’s multiple comparisons test. ***p<0.01, **** p<0.0001.